Falleroni 2018

2018 - Front Cell Neurosci. 2018 May 14;12:130. doi: 10.3389/fncel.2018.00130. eCollection 2018

Cell Mechanotransduction With Piconewton Forces Applied by Optical Tweezers

Falleroni F, Torre V, Cojoc D

Abstract:

Mechanical stresses are always present in the cellular environment and mechanotransduction occurs in all cells. Although many experimental approaches have been developed to investigate mechanotransduction, the physical properties of the mechanical stimulus have yet to be accurately characterized. Here, we propose a mechanical stimulation method employing an oscillatory optical trap to apply piconewton forces perpendicularly to the cell membrane, for short instants. We show that this stimulation produces membrane indentation and induces cellular calcium transients in mouse neuroblastoma NG108-15 cells dependent of the stimulus strength and the number of force pulses

 

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