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Giuliano Taccola

SPINAL
 
Spinal cord neurophysiology

Giuliano Taccola Researcher
 

Giuliano Taccola is an Assistant Professor in Pharmacology at the International School for Advanced Studies (SISSA) in Italy (ORCID: https://orcid.org/0000-0003-2675-1438). He is an established Principal Investigator, leading his own laboratory of Applied Neurophysiology and Neuropharmacology at the Regional Spinal Center in Udine and collaborating with scientists worldwide to study the physiology of spinal motoneurons, the development and plasticity of spinal networks, their pharmacological and electrical neuromodulation, as well as the cellular, circuit and systems mechanisms at the base of spinal cord injury and other neuromotor disorders.

Taccola’s lab has a vast experience on in vitro spinal cord preparations isolated from neonatal rodents, performing intracellular recordings from single motoneurons using microelectrodes, AC- and DC- coupled recordings from spinal roots and peripheral nerves, and whole cell patch clamp recordings from spinal inter- and moto- neurons performed in current/voltage clamp modes. Since 2015, he researches also on preclinical animal models with and without a spinal cord injury, in a still ongoing collaboration with UCLA and the University of Leeds, where he worked from 2015 to 2018 with a Marie Skłodowska-Curie Global Individual Fellowships (https://cordis.europa.eu/project/rcn/196101/factsheet/en). This line of research combines cutting-edge in vivo and in vitro electrophysiology, neuropharmacology, multi-electrode interfaces and neuroprosthetics, and behavioural neuroscience.

As part of this collaboration, Giuliano Taccola explores new pharmacological and electrical stimulating protocols to restore motor functions in preclinical rodent models of spinal cord injury.

In the coming years, in vivo electrophysiological and behavioural assessments in preclinical models will be associated to in vitro recordings from reduced spinal cord preparations in order to: 

 

Further define the biophysical membrane properties of motoneurons during development, the organization of neonatal interneuronal spinal networks generating rhythmic activity and their pharmacological modulation.

 

Taccola 1

 

 

 

 

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Explore the effects of passive training on spinal circuits, also comparing real locomotor training with a recently invented robotic device (BIKE, Bicycle Induced Kinetic Exercise) that reproduces a standardized hindlimb passive motion in the isolated spinal cord – leg attached preparation of neonatal rats.

 

Taccola 2

 

 

 

 

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Limit the acute consequences of a spinal cord injury and support the recovery of functions in chronic spinal cord injuries, by associating innovative electro-stimulating protocols, stochastically modulated in both frequency and amplitude (noisy), with the design of innovative multi-electrode epidural interfaces for independent multi-site stimulation and recordings, and combine them with new pharmacological interventions.

 

Taccola 3 

 

 

 

research summary

Neurophysiology and neuropharmacology of the spinal cord

The research interests of the laboratory are focused on the neurophysiology and neuropharmacology of the spinal cord, before and after lesion, with a particular emphasis on the functional organization of the neuronal circuits responsible for the generation of the locomotor rhythm (locomotor central pattern generator, CPG). The main scientific goal is to identify new experimental strategies to activate the CPG, in a perspective to propose new therapeutic interventions for the functional recovery of standing posture and deambulation after spinal cord injury.

Several innovative patterns of electrical stimulation have been devised in our lab by sampling the rhythmic noisy waves that appear during fictive or real motor patterns recorded, respectively, from the isolated neonatal rat spinal cord or from EMG recordings of lower limbs during real locomotion in healthy volunteers.

Their combination with pharmacological stimulation is a further aspect that our group is considering to synergize the performance of these protocol.

In collaboration with international partners, experts in preclinical rodent models, we are also assessing how different protocols of electrical stimulation or forced passive motor activity can modulate the hyperactivity of in vitro dorsal networks and thus alleviate the neuropathic pain associated with a spinal damage or with a lesion of the peripheral nerve.

The collaboration with young mathematicians is pursued in order to have available new methods of analysis to decipher how the noise contained in variable stimulating patterns can be beneficial to the activation of neuronal networks. Moreover, the development of always more sophisticated tools to quantify the degree of neuronal network activation is followed.

Joined projects with bioengineers are sponsored to develop new and more efficient devices for electrical stimulation.

The daily contact with clinical researchers of the IMFR supports the laboratory in focusing its studies towards the open questions in the neurorehabilitation of persons with a spinal cord injury and offers the possibility to translate the most promising observations obtained in our laboratory into clinics.

Selected publications

Mol Neurobiol. 2021 Apr 7. | Epub ahead of print. PMID: 33826070. | doi: 10.1007/s12035-021-02370-5. |

GABAergic Mechanisms Can Redress the Tilted Balance between Excitation and Inhibition in Damaged Spinal Networks

Mazzone GL, Mohammadshirazi A, Aquino JB, Nistri A, Taccola G.

Cell Mol Neurobiol. 2021 Jan;41(1):185-190. | Epub 2020 Mar 24. PMID: 32211996. | doi: 10.1007/s10571-020-00831-4. |

Histamine H3 Receptors Expressed in Ventral Horns Modulate Spinal Motor Output

Coslovich T, Della Mora A, D'Angelo G, Ortolani F, Taccola G.

Front. Syst. Neurosci., 14 July 2020 | https://doi.org/10.3389/fnsys.2020.00044

Selective Antagonism of A1 Adenosinergic Receptors Strengthens the Neuromodulation of the Sensorimotor Network During Epidural Spinal Stimulation

Taccola G, Salazar BH, Apicella R, Hogan MK, Horner PJ, Sayenko D.

Spinal Cord. 2020 Oct;58(10):1049-1059. | Epub 2020 Jun 23. PMID: 32576946. | doi: 10.1038/s41393-020-0505-8.

Complications of epidural spinal stimulation: lessons from the past and alternatives for the future

Taccola G, Barber S, Horner PJ, Bazo HAC, Sayenko D.

Brain Stimulation, Volume 13, Issue 1, January–February 2020, Pages 20-34 | https://doi.org/10.1016/j.brs.2019.09.013

Using EMG to deliver lumbar dynamic electrical stimulation to facilitate cortico-spinal excitability

Taccola G, Gad P, Culaclii S, Ichiyama RM, Liu W, Edgerton VR.
Experimental Neurology., Volume 327, May 2020, 113246 | https://doi.org/10.1016/j.expneurol.2020.113246

Acute neuromodulation restores spinally-induced motor responses after severe spinal cord injury

Taccola G, Gad P, Culaclii S, Wang PM, Liu W, Edgerton VR.
Pat. n. WO 2020/236946 A1

TRANSCUTANEOUS ELECTRICAL SPINAL CORD NEUROMODULATOR AND USES THEREOF

V Edgerton, G Taccola, E Kreydin

Experimental Cell Research. | Volume 381, Issue 1, 1 August 2019, Pages 121-128

A "noisy" electrical stimulation protocol favors muscle regeneration in vitro through release of endogenous ATP

Bosutti A, Bernareggi A, Massaria G, D'Andrea P, Taccola G; Lorenzon P, Sciancalepore M.

J Neurosci Res., 2018.

Histamine modulates spinal motoneurons and locomotor circuits

Coslovich T, Brumley MR, D’Angelo G, Della Mora A, Swann HE, Ortolani F, and Taccola G.

Prog Neurobiol., Volume 160, January 2018, Pages 64-81 | https://doi.org/10.1016/j.pneurobio.2017.10.004

And yet it moves: recovery of volitional control after spinal cord injury

Taccola G, Gerasimenko Y, Gad P, Sayenko D, and Edgerton VR.

Invited review to Current Pharmaceutical Design. | Volume 23, Issue 12, 2017

Multilevel analysis of locomotion in immature preparations suggests innovative strategies to reactivate stepping after spinal cord injury

Brumley MR, Guertin PA, Taccola G. 

Experimental Neurology., 2016

Neuromodulation of the neural circuits controlling the lower urinary tract

Gad P, Roy R, Zhong H, Gerasimenko Y, Taccola G, Edgerton VR.  

Experimental Neurology, Volume 286, December 2016, Pages 1-11 | https://doi.org/10.1016/j.expneurol.2016.09.008

A new model of nerve injury in the rat reveals a role of regulator of G protein signaling 4 in tactile hypersensitivity

Taccola G, Doyen PJ, Damblon J, Dingu N, Ballarin B, Steyaert A, des Rieux A, Forget P, Hermans E, Bosier B, Deumens R.  

J Muscle Res Cell Motil., 2015

Extracellular stimulation with human “noisy” electromyographic patterns facilitates myotube activity

Sciancalepore M, Coslovich T, Lorenzon P, Ziraldo G, Taccola G.  

Neuromethods 2013, 76, 39-62.

Acute Spinal Cord Injury In Vitro: Insight Into Basic Mechanisms.

Mladinic M, Nistri A, Taccola G.

Neuroscience. 2013 Nov 12;252:144-53.

Schwann cell migration and neurite outgrowth are influenced by media conditioned by epineurial fibroblasts

van Neerven SG, Pannaye P, Bozkurt A, Van Nieuwenhoven F, Joosten E, Hermans E, Taccola G, Deumens R.

Neuroscience, Volume 222, 11 October 2012, Pages 191-204 | https://doi.org/10.1016/j.neuroscience.2012.07.030

A1 adenosine receptor modulation of chemically and electrically evoked lumbar locomotor network activity in isolated newborn rat spinal cords

Taccola G, Olivieri D, D'Angelo G, Blackburn P, Secchia L, Ballany K.

 
 

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